Genotipagem da resistência genotípica secundária aos antirretrovirais em pacientes com aids nos Estados do Pará e Amazonas, Brasil: 2002 a 2006*

Olinda Macêdo; Luciana Macedo Ferreira; Pedro Fernando da Costa Vasconcelos; Rita Catarina Medeiros de Sousa; Carmen Andrea Freitas; José Ricardo Mourão Araújo

doi:10.5123/S2176-62232011000300004

Authors

Olinda Macêdo Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Luciana Macedo Ferreira Seção de Virologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Pedro Fernando da Costa Vasconcelos Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Rita Catarina Medeiros de Sousa Núcleo de Medicina Tropical, Universidade Federal do Pará, Belém, Pará, Brasil
Carmen Andrea Freitas Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, Pará, Brasil
José Ricardo Mourão Araújo Casa Dia, Secretária Municipal de Saúde, Belém, Pará, Brasil

DOI:

https://doi.org/10.5123/S2176-62232011000300004

Keywords:

Terapia Anti-Retroviral, VIH-1, Mutation, Genotyping

Abstract

ABSTRACT

Resistance to antiretroviral drugs results from the incomplete suppression of HIV-1 replication. The present study characterized the profile of genotypic resistance to antiretroviral drugs (ARVs) in serum samples from 127 HIV-positive patients from the States of Amazonas and Pará, in Northern Brazil, from 2002 to 2006. The samples were tested for resistance using the ViroSeq^TM Genotyping System kit. Based on the genetic information obtained from the HIV-1 protease (PR) and/or reverse transcriptase (RT) genes, the M184V mutation (81.1%) was the most frequently associated with resistance to nucleoside reverse transcriptase inhibitors (NRTIs) in individuals using ARVs in Pará, while the T215F/Y mutation (56.3%) was the most frequently associated with resistance in individuals from Amazonas. The K103N mutation was the most prevalent (33.5%) resistance mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) in both states. For the PR gene, the minor mutation L63P (65.3%) was the most frequent in both states. The present study showed the importance of identifying mutations associated with resistance to ARVs to the rational selection of therapeutic schemes. Additionally, the results found in Pará and Amazonas were found to be similar to those of other areas in Brazil.