Resistência a antimicrobianos de enterobactérias isoladas de águas destinadas ao abastecimento público na região centro-oeste do estado de São Paulo, Brasil

André Martins; Rosângela Aguilar Silva; Luci Occhi Ferreira; Marina Madalena Licate; Cláudia Regina Delafiori; Salete França Pôrto

doi:10.5123/S2176-6223201900065

Authors

André Martins Instituto Adolfo Lutz, Núcleo de Ciências Biomédicas, Marília, São Paulo, Brasil http://orcid.org/0000-0002-1857-2248
Rosângela Aguilar Silva Instituto Adolfo Lutz, Núcleo de Ciências Químicas e Bromatológicas, Marília, São Paulo, Brasil http://orcid.org/0000-0002-1249-447X
Luci Occhi Ferreira Instituto Adolfo Lutz, Núcleo de Ciências Químicas e Bromatológicas, Marília, São Paulo, Brasil http://orcid.org/0000-0001-6727-0898
Marina Madalena Licate Instituto Adolfo Lutz, Núcleo de Ciências Químicas e Bromatológicas, Marília, São Paulo, Brasil http://orcid.org/0000-0003-3509-7132
Cláudia Regina Delafiori Instituto Adolfo Lutz, Núcleo de Ciências Biomédicas, Marília, São Paulo, Brasil http://orcid.org/0000-0003-0418-4655
Salete França Pôrto Instituto Adolfo Lutz, Núcleo de Ciências Biomédicas, Marília, São Paulo, Brasil http://orcid.org/0000-0003-1952-8537

DOI:

https://doi.org/10.5123/S2176-6223201900065

Keywords:

Anti-Bacterial Agents, Bacterial Drug Resistance, Drinking Water, Enterobacteriaceae

Abstract

ABSTRACT

OBJECTIVES:

To isolate and identify antimicrobial-resistant enterobacteria from drinking water in the Midwest region of São Paulo State, Brazil.

MATERIALS AND METHODS:

A total of 3,726 water samples from public supply system and collective wells from 62 municipalities were analyzed. Positive samples for total coliforms and/or Escherichia coli were seeded on MacConkey Agar for isolation and identification. Isolated enterobacteria were evaluated for antimicrobial resistance detection and expanded spectrum β-lactamase production.

RESULTS:

From a total of 67 water samples from 29 municipalities with growth of enterobacteria, 21 (31.34%) were resistant to at least one antimicrobial, seven (10.43%) to two classes of antimicrobials, three (4.47%) to three classes, and one (1.49%) to four classes. The drug with the highest number of resistant bacterial samples was cefoxitin (12), followed by cephalothin (eight), cefotaxime (five), ticarcillin + clavulanate (four), aztreonam (four), nalidixic acid (three), and ampicillin + sulbactam (three). Second generation cephalosporins showed the largest number of resistant bacterial samples (12; 32.43%), followed by penicillins (10; 14.92%), and first generation cephalosporins (8; 25.81%). Three (4.47%) samples were resistant to three classes of antimicrobials and one (1.49%) to four classes.

CONCLUSION:

Drinking water can be a source of spread of resistant and multiresistant Gram-negative bacteria, characterizing a public health problem. Proper water treatment and monitoring of public water supply systems are important to detect antimicrobial resistant bacteria.

Keywords: Anti-Bacterial Agents; Bacterial Drug Resistance; Drinking Water; Enterobacteriaceae