Bioensaio com Allium cepa revela genotoxicidade de herbicida com flumioxazina

Lais Gonçalves Parvan; Thaís Gonçalves Leite; Thaynara Barbosa Freitas; Polyana Aparecida Almeida Pedrosa; Juliana Sena Calixto; Luciana de Andrade Agostinho

doi:10.5123/S2176-6223202000544

Authors

Lais Gonçalves Parvan Centro Universitário UNIFAMINAS, Muriaé, Minas Gerais, Brasil http://orcid.org/0000-0001-7684-3686
Thaís Gonçalves Leite Centro Universitário UNIFAMINAS, Muriaé, Minas Gerais, Brasil http://orcid.org/0000-0001-5021-5163
Thaynara Barbosa Freitas Centro Universitário UNIFAMINAS, Muriaé, Minas Gerais, Brasil http://orcid.org/0000-0002-3887-8703
Polyana Aparecida Almeida Pedrosa Centro Universitário UNIFAMINAS, Muriaé, Minas Gerais, Brasil http://orcid.org/0000-0002-0564-8207
Juliana Sena Calixto Instituto Federal de Educação, Ciência e Tecnologia do Sudeste de Minas Gerais, Muriaé, Minas Gerais, Brasil http://orcid.org/0000-0003-0810-7817
Luciana de Andrade Agostinho Centro Universitário UNIFAMINAS, Muriaé, Minas Gerais, Brasil; Fundação Cristiano Varella, Hospital do Câncer de Muriaé, Muriaé, Minas Gerais, Brasil; Universidade Federal http://orcid.org/0000-0001-5604-8386

DOI:

https://doi.org/10.5123/S2176-6223202000544

Keywords:

Genotoxicity, Allium cepa, Herbicides, Mitotic Index

Abstract

ABSTRACT

OBJECTIVE:

To analyze the genotoxic potential of an herbicide with flumioxazin using the Allium cepa bioassay.

MATERIALS AND METHODS:

The herbicide was tested in two concentrations: 0.5 g/L (as indicated for use) and 1.0 g/L (twice the recommended concentration). Distilled water was used as a negative control and 1% sodium benzoate as a positive cytotoxicity control.

RESULTS:

Toxicity effects were evidenced in both herbicide concentrations. Cytotoxicity effects were observed in the herbicide with flumioxazin at a concentration of 0.5 g/L, which showed a microscopic pattern equal to that of the positive control. The effects of genotoxicity were observed at both concentrations (0.5 g/L and 1.0 g/L) which resulted in chromosomal aberrations in cells, showing that the herbicide directly interferes with the cell cycle of vegetal onion cells.

CONCLUSION:

The herbicide with flumioxazin showed toxicity and genotoxicity in the analyzed concentrations and effects of cytotoxicity only in the concentration recommended for use.