Susceptibility of a continuous murine cell line (GRX) to viral infection

Maria Liz Gamarra; Maria Carolina Maciel Albuquerque; Anderson Junger Teodoro; Renata Brum Martucci; Radovan Borojevic; Fernando Portela Câmara; Maria Teresa Villela Romanos; Norma Santos

doi:10.5123/S2176-62232011000200009

Authors

Maria Liz Gamarra Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil
Maria Carolina Maciel Albuquerque Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil
Anderson Junger Teodoro Departamento de Histologia, Instituto de Ciências Biológicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil
Renata Brum Martucci Departamento de Nutrição Aplicada, Instituto de Nutrição, Universidade Estadual do Rio de Janeiro, Rio de Janeiro, Brasil Endereço para correspondência
Radovan Borojevic Departamento de Histologia, Instituto de Ciências Biológicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil
Fernando Portela Câmara Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil
Maria Teresa Villela Romanos Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil
Norma Santos Departamento de Virologia, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brasil

DOI:

https://doi.org/10.5123/S2176-62232011000200009

Keywords:

Cell culture technique, Hepatic Stellate Cells (GRX), Virus replication

Abstract

ABSTRACT

The ability of a murine cell line (GRX) to support viral replication was evaluated. GRX cell cultures were infected with different DNA or RNA viruses. It was observed that the GRX cell line is susceptible to the replication of Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), Mayaro virus (MAY), Sindbis virus (SIN), and West equine encephalitis virus (WEE), and can be used as substrate for viral replication studies. Viral replication induced cytopathic effect (CPE) 24-48 h post-infection. The GRX cells yielded infectious virus titers between 10^2.4 TCID₅₀ (Tissue Culture Infectious Dose₅₀) /25 µL and 10^5.4 TCID₅₀/25 µL in the first viral passage. These results demonstrate that GRX cells efficiently sustain viralreplication and therefore can be used as a valuable tool in the virology laboratory.