Molecular epidemiology of dengue virus serotypes 2 and 3 isolated in Brazil from 1991 to 2008

Ana Cecília Ribeiro Cruz; Ricardo Galler; Eliana Vieira Pinto da Silva; Mayra de Oliveira e Silva; Adriana Ribeiro Carneiro; Elizabeth Salbé Travassos da Rosa; Helena Baldez Vasconcelos; Eric Luiz Rodrigues de Sá; Pedro Fernando da Costa Vasconcelos

doi:10.5123/S2176-62232010000300004

Authors

Ana Cecília Ribeiro Cruz Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro
Ricardo Galler Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brasil
Eliana Vieira Pinto da Silva Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Mayra de Oliveira e Silva Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Adriana Ribeiro Carneiro Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Elizabeth Salbé Travassos da Rosa Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Helena Baldez Vasconcelos Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil
Eric Luiz Rodrigues de Sá Fundação de Medicina Tropical do Tocantins, Araguaina, Tocantins, Brasil
Pedro Fernando da Costa Vasconcelos Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brasil

DOI:

https://doi.org/10.5123/S2176-62232010000300004

Keywords:

Dengue Virus, Dengue Hemorrhagic Fever, Flavivirus

Abstract

ABSTRACT

The dengue virus (DENV1-4) causes dengue fever and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) in tropical and subtropical areas. The aim of this study was to evaluate the circulating genotypes of DENV. This was accomplished by sequencing the PrM and E genes of Brazilian isolates of DENV2 and DENV3 that were obtained between 1991 and 2008 from various geographic regions. Phylogenetic analyses of DENV2 demonstrated that the genotype III (Southeast Asian/American), in spite of several nucleotide and amino acid changes, was the only one that circulated over the past 19 years. Since its introduction in 2000, the DENV3 isolates that have been analyzed have all grouped into genotype III (Indian subcontinent) and there has been no evidence of DENV3 belonging to other genotypes in this study.